The trio have recently been sidelined by injuries and none are ready to return in time for Tuesday night’s Champions League fixture at Anfield.
Alisson and Frimpong will also be absent for the subsequent Premier League trip to Manchester City at the weekend, though Isak has a chance of making the squad.
“All three are not available for tomorrow,” head coach Slot told his pre-Real press conference on Monday.
“Jeremie and Alisson will both also not be available for Sunday.
“With Alex we have to wait and see. Definitely not starting on Sunday, but maybe he can be involved in the squad.
“He is still not training with the team, so we have to wait and see.”