Emergency services were alerted to an aggravated assault at a home in Laramba, a town with a population of around 275 people 205km north-west of Alice Springs, at 4.40am today.
They found the man seriously injured and rushed him to a clinic for treatment, but he died.
Officers from neighbouring towns and Alice Springs have travelled to Laramba and set up a crime scene.
Detectives from the major crime unit are also on their way.
Investigations are ongoing.