The FBI said on Sunday that investigators recovered the glove from a field near the side of the road about two miles (3 km) from Guthrie’s home near Tucson, Arizona.
The agency collected about 16 gloves from various locations near Nancy Guthrie’s home, although most turned out to have been discarded by people searching for her.
Last week, investigators released video footage taken from a doorbell camera at Guthrie’s front door that showed a masked man wearing gloves and a backpack.
In the video, the suspect walks toward the camera and tries to cover it with his gloved hand before grabbing some brush from the front yard to block the camera’s view.
After conducting a forensic analysis of the video, the FBI said it believed the man was between 5ft 9in and 5ft 10in (175-177cm) tall with an average build.
More than two weeks since Guthrie was reported missing, no arrests have been made, the sheriff’s department confirmed on Tuesday, adding that more DNA found in the home is being analysed.
Authorities also shared that they are working with Walmart to determine who purchased the backpack worn by the suspect in the video.