Petrowsky, H. et al. Modern therapeutic approaches for the treatment of malignant liver tumours. Nat. Rev. Gastroenterol. Hepatol. 17, 755–772 (2020).

PubMed 

Google Scholar
 

Huang, M., Lu, J. J. & Ding, J. Natural products in cancer therapy: Past, present and future. Nat. Prod. Bioprospect. 11, 5–13 (2021).

PubMed 
PubMed Central 

Google Scholar
 

Attia, A. A. et al. Amygdalin potentiates the anti-cancer effect of Sorafenib on Ehrlich Ascites carcinoma and ameliorates the associated liver damage. Sci. Rep. 12, 1–9 (2022).


Google Scholar
 

Chi, H. et al. Characterization of a novel and glutaminase-free type II L-asparaginase from Corynebacterium glutamicum and its acrylamide alleviation efficiency in potato chips. Int. J. Biol. Macromol. 221, 1384–1393 (2022).

PubMed 

Google Scholar
 

Zhou, Y. et al. Rational engineering and insight for a L-glutaminase activity reduced type II L-asparaginase from Bacillus licheniformis and its antileukemic activity in vitro. Int. J. Biol. Macromol. 257, 128690 (2024).

PubMed 

Google Scholar
 

Barzkar, N., Sohail, M., Tamadoni Jahromi, S., Nahavandi, R. & Khodadadi, M. Marine microbial L-glutaminase: from pharmaceutical to food industry. Appl. Microbiol. Biotechnol. 105, 4453–4466 (2021).

PubMed 

Google Scholar
 

Debnath, T., Kujur, R. R. A., Mitra, R. & Das, S. K. Diversity of microbes in hot springs and their sustainable use. Microb. Divers. Ecosyst. Sustain. Biotechnol. Appl. Vol. 1. Microb. Divers. Norm. Extrem. Environ. 159–186 (2019).

Haki, G. D. & Rakshit, S. K. Developments in industrially important thermostable enzymes: A review. Bioresour Technol. 89, 17–34 (2003).

PubMed 

Google Scholar
 

Feller, G. Protein stability and enzyme activity at extreme biological temperatures. J. Phys. Condens. Matter. 22, 323101 (2010).

PubMed 

Google Scholar
 

Nigam, P. S. Microbial enzymes with special characteristics for biotechnological applications. Biomolecules 3, 597–611 (2013).

PubMed 
PubMed Central 

Google Scholar
 

Sanchez, S. & Demain, A. L. Enzymes and bioconversions of industrial, pharmaceutical, and biotechnological significance. Org. Process. Res. Dev. 15, 224–230 (2011).


Google Scholar
 

Kumar, A., Mukhia, S. & Kumar, R. Industrial applications of cold-adapted enzymes: Challenges, innovations and future perspective. 3 Biotech. 11, 426 (2021).

PubMed 
PubMed Central 

Google Scholar
 

Lukey, M. J., Wilson, K. F. & Cerione, R. A. Therapeutic strategies impacting cancer cell glutamine metabolism. Future Med. Chem. 5, 1685–1700 (2013).

PubMed 

Google Scholar
 

Nguyen, H. A., Su, Y. & Lavie, A. Design and characterization of erwinia chrysanthemi L-asparaginase variants with diminished L-glutaminase activity. J. Biol. Chem. 291, 17664–17676 (2016).

PubMed 
PubMed Central 

Google Scholar
 

Orabi, H., El-Fakharany, E., Abdelkhalek, E. & Sidkey, N. Production, optimization, purification, characterization, and anti-cancer application of extracellular L-glutaminase produced from the marine bacterial isolate. Prep Biochem. Biotechnol. 50, 408–418 (2020).

PubMed 

Google Scholar
 

Maurya, D. K., Kumar, A., Chaurasiya, U., Hussain, T. & Singh, S. K. Modern era of microbial biotechnology: opportunities and future prospects. in Microbiomes and plant health 317–343Elsevier, (2021).

Fidelito, G. et al. Multi-substrate metabolic tracing reveals marked heterogeneity and dependency on fatty acid metabolism in human prostate cancer. Mol. Cancer Res. 21, 359–373 (2023).

PubMed 

Google Scholar
 

Pandian, S. R. K., Deepak, V., Nellaiah, H. & Sundar, K. PEG–PHB-glutaminase nanoparticle inhibits cancer cell proliferation in vitro through glutamine deprivation. Vitr Cell. Dev. Biol. 51, 372–380 (2015).


Google Scholar
 

Kuo, M. T., Chen, H. H. W., Feun, L. G. & Savaraj, N. Targeting the proline–glutamine–asparagine–arginine metabolic axis in amino acid starvation cancer therapy. Pharmaceuticals 14, 72 (2021).

PubMed 
PubMed Central 

Google Scholar
 

Carneiro, B. A. & El-Deiry, W. S. Targeting apoptosis in cancer therapy. Nat. Rev. Clin. Oncol. 17, 395–417 (2020).

PubMed 
PubMed Central 

Google Scholar
 

Elmetwalli, A. et al. Diarylheptanoids/sorafenib as a potential anticancer combination against hepatocellular carcinoma: the p53/MMP9 axis of action. Naunyn Schmiedebergs Arch. Pharmacol 1–17 (2023).

Matés, J. M. et al. Metabolic reprogramming of cancer by chemicals that target glutaminase isoenzymes. Curr. Med. Chem. 27, 5317–5339 (2020).

PubMed 

Google Scholar
 

Kao, T. W., Chuang, Y. C., Lee, H. L., Kuo, C. C. & Shen, Y. A. Therapeutic targeting of Glutaminolysis as a novel strategy to combat cancer stem cells. Int. J. Mol. Sci. 23, 15296 (2022).

PubMed 
PubMed Central 

Google Scholar
 

Kumar, M. et al. Identification of small molecule inhibitors of RAD52 for breast cancer therapy: in Silico approach. J. Biomol. Struct. Dyn. 42, 4605–4618 (2024).

PubMed 

Google Scholar
 

Gupta, D. et al. A comprehensive review on role of Aurora kinase inhibitors (AKIs) in cancer therapeutics. Int J. Biol. Macromol 130913 (2024).

Gupta, D. et al. Identification of polypharmacological anticancerous molecules against Aurora kinase family of proteins. J. Cell. Biochem. 123, 719–735 (2022).

PubMed 

Google Scholar
 

Agu, P. C. et al. Molecular Docking as a tool for the discovery of molecular targets of nutraceuticals in diseases management. Sci. Rep. 13, 13398 (2023).

ADS 
PubMed 
PubMed Central 

Google Scholar
 

Elmetwalli, A. et al. Evaluation of Bacillus Aryabhattai B8W22 peroxidase for phenol removal in waste water effluents. BMC Microbiol. 23, 1–13 (2023).


Google Scholar
 

Hasan, S. F., Elsoud, M. M. A., Sidkey, N. M. & Elhateir, M. M. Production and characterization of polyhydroxybutyrate bioplastic precursor from Parageobacillus toebii using low-cost substrates and its potential antiviral activity. Int J. Biol. Macromol 129915 (2024).

El-Hadedy, D. E., MH, S., HH, E. & Mm, S. Enhanced production of extracellular L-methioninase by entire cell immobilization of streptomyces MDMMH4 and examination of its utilization as an antioxidant agent. Pak J. Pharm. Sci 36, (2023).

Abellan-Schneyder, I. et al. Primer, pipelines, parameters: Issues in 16S rRNA gene sequencing. Msphere 6, 10–1128 (2021).


Google Scholar
 

Abdallah, N. A., Amer, S. K. & Habeeb, M. K. Production, purification and characterization of L-glutaminase enzyme from streptomyces avermitilis. Afr. J. Microbiol. Res. 14, 1184–1190 (2013).


Google Scholar
 

Katikala, P. K., Bobbarala, V., Tadimalla, P. & Guntuku, G. S. Screening of L-glutaminase producing marine bacterial cultures for extracellular production of L-glutaminase. Int. J. ChemTech Res. 1, 1232–1235 (2009).


Google Scholar
 

Jayabalan, R. et al. Extracellular L-glutaminase production by marine brevundimonas diminuta MTCC 8486. Int. J. Appl. Bioeng. 4, 19–24 (2010).


Google Scholar
 

Reda, F. M. Kinetic properties of streptomyces Canarius L-Glutaminase and its anticancer efficiency. Brazilian J. Microbiol. 46, 957–968 (2015).


Google Scholar
 

El-Sewedy, T. et al. Hepatocellular carcinoma cells: Activity of amygdalin and Sorafenib in targeting ampk/mtor and BCL-2 for anti-angiogenesis and apoptosis cell death. BMC Complement. Med. Ther. 23, 1–17 (2023).


Google Scholar
 

Elmetwalli, A. et al. Probiotic-derived silver nanoparticles target mTOR/MMP-9/BCL-2/dependent AMPK activation for hepatic cancer treatment. Med. Oncol. 41, 106 (2024).

PubMed 
PubMed Central 

Google Scholar
 

El-Shehawy, A. A. et al. Thymoquinone, piperine, and Sorafenib combinations attenuate liver and breast cancers progression: Epigenetic and molecular Docking approaches. BMC Complement. Med. Ther. 23, 1–21 (2023).


Google Scholar
 

Al Balawi, A. N., Eldiasty, J. G., Mosallam, S. A. E. R., El-Alosey, A. R. & Elmetwalli, A. Assessing multi-target antiviral and antioxidant activities of natural compounds against SARS-CoV-2: an integrated in vitro and in Silico study. Bioresour Bioprocess. 11, 108 (2024).

PubMed 
PubMed Central 

Google Scholar
 

Vilar, S., Cozza, G. & Moro, S. Medicinal chemistry and the molecular operating environment (MOE): Application of QSAR and molecular Docking to drug discovery. Curr. Top. Med. Chem. 8, 1555–1572 (2008).

PubMed 

Google Scholar
 

Bewick, V., Cheek, L. & Ball, J. Statistics review 9: One-way analysis of variance. Crit. Care. 8, 1–7 (2004).


Google Scholar
 

Rafeeq, H. et al. Esterases as emerging biocatalysts: mechanistic insights, genomic and metagenomic, immobilization, and biotechnological applications. Biotechnol. Appl. Biochem. 69, 2176–2194 (2022).

PubMed 

Google Scholar
 

Tandon, S., Sharma, A., Singh, S., Sharma, S. & Sarma, S. J. Therapeutic enzymes: Discoveries, production and applications. J. Drug Deliv Sci. Technol. 63, 102455 (2021).


Google Scholar
 

Binod, P. et al. Recent developments in l-glutaminase production and applications–An overview. Bioresour Technol. 245, 1766–1774 (2017).

PubMed 

Google Scholar
 

Srinivasan, P. et al. Production and purification of laccase by Bacillus sp. using millet husks and its pesticide degradation application. 3 Biotech. 9, 396 (2019).

PubMed 
PubMed Central 

Google Scholar
 

Gomaa, E. Z. Production, characterization, and antitumor efficiency of l-glutaminase from halophilic bacteria. Bull. Natl. Res. Cent. 46, 10 (2022).


Google Scholar
 

Mostafa, Y. S. et al. L-glutaminase synthesis by marine Halomonas Meridiana isolated from the red sea and its efficiency against colorectal cancer cell lines. Molecules 26, 1963 (2021).

PubMed 
PubMed Central 

Google Scholar
 

Mousumi, D. & Dayanand, A. Production and antioxidant attribute of L-glutaminase from streptomyces enissocaesilis DMQ-24. Int. J. Latest Res. Sci. Technol. 2, 1–9 (2013).


Google Scholar
 

Gehlot, P., Kumar, M. & Pareek, N. Production and purification of glutaminase free L-asparaginase from Lysinibacillus fusiformis and its appraisal in acrylamide mitigation of starchy foods. Mater. Today Proc. 69, 64–73 (2022).


Google Scholar
 

Abhini, K. N., Rajan, A. B., Zuhara, K. F. & Sebastian, D. Response surface methodological optimization of l-asparaginase production from the medicinal plant endophyte acinetobacter baumannii ZAS1. J. Genet. Eng. Biotechnol. 20, 22 (2022).

PubMed 
PubMed Central 

Google Scholar
 

Arévalo-Tristancho, E., Díaz, L. E., Cortázar, J. E. & Valero, M. F. Production and characterization of L-Asparaginases of isolated from the Arauca riverbank (Colombia). Open Microbiol. J 13, (2019).

Wang, B., Pei, J., Xu, S., Liu, J. & Yu, J. A glutamine tug-of-war between cancer and immune cells: Recent advances in unraveling the ongoing battle. J. Exp. Clin. Cancer Res. 43, 74 (2024).

PubMed 
PubMed Central 

Google Scholar
 

Tang, X. et al. Selective enhanced cytotoxicity of amino acid deprivation for cancer therapy using thermozyme functionalized nanocatalyst. J. Nanobiotechnol. 22, 53 (2024).


Google Scholar
 

Srivastava, N., Srivastava, M., Mishra, P. K., Ramteke, P. W. & Singh, R. L. New and Future Developments in Microbial Biotechnology and Bioengineering: from Cellulose To Cellulase: Strategies To Improve Biofuel Production (Elsevier, 2019).

Ariaeenejad, S. et al. Highly efficient computationally derived novel metagenome α-amylase with robust stability under extreme denaturing conditions. Front. Microbiol. 12, 713125 (2021).

PubMed 
PubMed Central 

Google Scholar
 

Banerjee, S., Maiti, T. K. & Roy, R. N. Production, purification, and characterization of cellulase from acinetobacter Junii GAC 16.2, a novel cellulolytic gut isolate of Gryllotalpa africana, and its effects on cotton fiber and sawdust. Ann. Microbiol. 70, 1–16 (2020).


Google Scholar
 

Guajardo, N. & Schrebler, R. A. Upstream and downstream bioprocessing in enzyme technology. Pharmaceutics 16, 38 (2023).

PubMed 
PubMed Central 

Google Scholar
 

Amiri, S., Mokarram, R. R., Khiabani, M. S., Bari, M. R. & Khaledabad, M. A. Characterization of antimicrobial peptides produced by Lactobacillus acidophilus LA-5 and bifidobacterium lactis BB-12 and their inhibitory effect against foodborne pathogens. Lwt 153, 112449 (2022).


Google Scholar
 

Priyadarshini, A., Sahoo, M. M., Raut, P. R., Mahanty, B. & Sahoo, N. K. Kinetic modelling and process engineering of phenolics microbial and enzymatic biodegradation: a current outlook and challenges. J. Water Process. Eng. 44, 102421 (2021).


Google Scholar
 

Bhatt, P. et al. New insights into the degradation of synthetic pollutants in contaminated environments. Chemosphere 268, 128827 (2021).

PubMed 

Google Scholar
 

Qeshmi, F. I., Homaei, A., Fernandes, P. & Javadpour, S. Marine microbial L-asparaginase: Biochemistry, molecular approaches and applications in tumor therapy and in food industry. Microbiol. Res. 208, 99–112 (2018).


Google Scholar
 

Saleem, R. & Ahmed, S. Characterization of a new L-Glutaminase produced by achromobacter xylosoxidans RSHG1, isolated from an expired hydrolyzed L-Glutamine sample. Catalysts 11, 1262 (2021).


Google Scholar
 

Bagewadi, Z. K. et al. Molecular expression, purification and structural characterization of Recombinant L-Glutaminase from streptomyces Roseolus. Int. J. Biol. Macromol. 273, 133142 (2024).

PubMed 

Google Scholar
 

Mosallatpour, S., Aminzadeh, S., Shamsara, M. & Hajihosseini, R. Novel halo-and thermo-tolerant Cohnella sp. A01 L-glutaminase: Heterologous expression and biochemical characterization. Sci. Rep. 9, 19062 (2019).

ADS 
PubMed 
PubMed Central 

Google Scholar
 

Beckett, A. & Gervais, D. What makes a good new therapeutic L-asparaginase? World J. Microbiol. Biotechnol. 35, 1–13 (2019).


Google Scholar
 

Akram, F. et al. Abridgement of microbial esterases and their eminent industrial endeavors. Mol Biotechnol 1–17 (2024).

Sodhi, A. S. et al. Insights on sustainable approaches for production and applications of value added products. Chemosphere 286, 131623 (2022).

PubMed 

Google Scholar
 

Diwan, D. et al. Elsevier,. Microbial cancer therapeutics: A promising approach. in Seminars in cancer biology vol. 86 931–950 (2022).

Sawant, S. S., Patil, S. M., Gupta, V. & Kunda, N. K. Microbes as medicines: Harnessing the power of bacteria in advancing cancer treatment. Int. J. Mol. Sci. 21, 7575 (2020).

PubMed 
PubMed Central 

Google Scholar
 

Awad, M. F., El-Shenawy, F. S., El-Gendy, M. M. A. A. & El-Bondkly, E. A. M. Purification, characterization, and anticancer and antioxidant activities of l-glutaminase from Aspergillus versicolor Faesay4. Int. Microbiol. 24, 169–181 (2021).

PubMed 

Google Scholar
 

Abu-Tahon, M. A. & Isaac, G. S. Purification, characterization and anticancer efficiency of L-glutaminase from Aspergillus flavus. J. Gen. Appl. Microbiol. 65, 284–292 (2019).

PubMed 

Google Scholar
 

Matés, J. M., Campos-Sandoval, J. A. & de Los Santos-Jiménez, J. M rquez, J. Dysregulation of glutaminase and glutamine synthetase in cancer. Cancer Lett. 467, 29–39 (2019).

PubMed 

Google Scholar
 

Hassan, F. S. et al. Comprehensive insight into exploring the potential of microbial enzymes in cancer therapy: progress, challenges, and opportunities: A review. Int J. Biol. Macromol 134535 (2024).

Crosby, H. A. & Miller, K. E. Evaluating the analgesic effect of the GLS inhibitor 6-Diazo-5-Oxo-L-Norleucine in vivo. Pharm Pharmacol. Int. J 3, (2016).

Mendiratta, S. S., Sekulic, N., Lavie, A. & Colley, K. J. Specific amino acids in the first fibronectin type III repeat of the neural cell adhesion molecule play a role in its recognition and polysialylation by the polysialyltransferase ST8Sia IV/PST. J. Biol. Chem. 280, 32340–32348 (2005).

PubMed 

Google Scholar
 

pez-Gil, L., n, C. I., Téllez-Jurado, A., Velasco-Vel zquez, M. A. & Anducho-Reyes, M. A. Identification and analysis of anticancer therapeutic targets from the polysaccharide Krestin (PSK) and polysaccharopeptide (PSP) using inverse Docking. Molecules 29, 5390 (2024).


Google Scholar
 

Mates, J. M. et al. Glutaminase isoenzymes as key regulators in metabolic and oxidative stress against cancer. Curr. Mol. Med. 13, 514–534 (2013).

PubMed 

Google Scholar
 

Yang, W. H., Qiu, Y., Stamatatos, O., Janowitz, T. & Lukey, M. J. Enhancing the efficacy of glutamine metabolism inhibitors in cancer therapy. Trends Cancer. 7, 790–804 (2021).

PubMed 
PubMed Central 

Google Scholar
 

Elmetwalli, A. et al. Ammonia scavenger and glutamine synthetase inhibitors cocktail in targeting mTOR/β-catenin and MMP-14 for nitrogen homeostasis and liver cancer. Med. Oncol. 41, 38 (2023).

PubMed 

Google Scholar
 

Youness, A., Kamel, R., Elkasabgy, R. A., Shao, N., Farag, A. & P. & Recent advances in Tannic acid (gallotannin) anticancer activities and drug delivery systems for efficacy improvement; a comprehensive review. Molecules 26, 1486 (2021).


Google Scholar
 

Cai, Y. et al. Recent advances in anticancer activities and drug delivery systems of tannins. Med. Res. Rev. 37, 665–701 (2017).

PubMed 

Google Scholar
 

Ghasemian, M. et al. Recent progress in Tannic acid based approaches as a natural polyphenolic biomaterial for cancer therapy: A review. Biomed. Pharmacother. 166, 115328 (2023).

PubMed 

Google Scholar