Ms Stitt said she would be keeping PC Prior and the nine other colleagues who had died in her mind as she cycled.
“I think if we have that at the back of our memory, the pain of sitting in a saddle for two hundred miles over the next couple of days won’t be there,” she said.
“It’s really about the people, it’s about their legacy, and it’s forever about supporting their family and their friends through the charity Care of Police Survivors.”
The challenge will take the hundreds of cyclists through Worksop and Tamworth, ending with the national memorial service in Staffordshire.
There, the wristbands will be placed on the memorial in the presence of family members and friends of officers from across the UK.
The ride aims to help raise awareness of the Care of Police Survivors charity, which supports the families of police staff who have died.