From genotype to phenotype with 1,086 near telomere-to-telomere yeast genomes
Strain culture and DNA extraction We used a collection of S. cerevisiae isolates that were previously sequenced using…
Fueling chromosomal gene diversification and artificial evolution with CRISPR | Genome Biology
Wilson DS, Keefe AD. Random mutagenesis by PCR. Curr Protoc Mol Biol. 2001;51:8–3. Google Scholar Reetz MT, Wu…
Clarifying Uncertain Genetic Tests in Cancer Treatment
Cancer treatment is becoming more personalised. By considering a patient’s unique genetic and molecular profile, along with their…
Methods and applications of in vivo CRISPR screening
Auton, A. et al. A global reference for human genetic variation. Nature 526, 68–74 (2015). Google Scholar GarcÃa-GarcÃa,…
Precisely defining disease variant effects in CRISPR-edited single cells
Cell-line cultures and genomic editing HH cutaneous T cell lines (ATCC, CRL-2105), Jurkat E6-1 (ATCC, TIB-152) and Daudi…