Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing
Adapting prime editing for mammalian histone mutagenesis Mouse canonical histone H3 is encoded by nine H3.1 and three…
Spatially resolved single-cell analyses of human meningioma identify novel cell states influencing tumor microenvironment and progression
Study cohort Our cohort focused on snRNA-seq (n = 102 samples and 580,185 high-quality nuclei) and spatial transcriptomics (Visium HD,…
The promise of adaptive health in the United Arab Emirates and beyond
Department of Medicine, Johns Hopkins University, Baltimore, MD, USA Andrew P. Feinberg Department of Biomedical Engineering, Johns Hopkins…
Pleiotropic effects of cis-regulatory mutations
Wray, G. A. The evolutionary significance of cis-regulatory mutations. Nat. Rev. Genet. 8, 206–216 (2007). Article CAS PubMed …
Evolutionary causes and consequences of gene duplication
Kuzmin, E., Taylor, J. S. & Boone, C. Retention of duplicated genes in evolution. Trends Genet. 38, 59–72…
Harnessing artificial intelligence to advance CRISPR-based genome editing technologies
Zheng, Y. et al. Precise genome-editing in human diseases: mechanisms, strategies and applications. Signal. Transduct. Target. Ther. 9,…
Pathogenic UNC13A variants cause a neurodevelopmental syndrome by impairing synaptic function
Institute of Medical Genetics, University of Zurich, Zurich, Switzerland Reza Asadollahi, Paranchai Boonsawat, Dennis Kraemer & Anita Rauch Faculty of Engineering and…
Modeling heterogeneity in single-cell perturbation states enhances detection of response eQTLs
Cohorts We used published data (GEO accession no. GSE162632) to study the effect of 12 h of ex vivo…
Genotyping sequence-resolved copy number variation using pangenomes reveals paralog-specific global diversity and expression divergence of duplicated genes
Overview of the genotyping method We represent variation as haplotype segments that are short enough to minimize disruption…
Redefining cellular reprogramming with advanced genomic technologies
Briggs, R. & King, T. J. Transplantation of living nuclei from blastula cells into enucleated Frogs’ eggs. Proc.…
Meta-analysis reveals differences in somatic alterations by genetic ancestry across common cancers
Genetic ancestry is a quantitative measure of inherited genetic variation and correlates with human migration patterns1. It contributes…
Population-scale gene-based analysis of whole-genome sequencing provides insights into metabolic health
Ethics Our research complies with all relevant ethical regulations. All studies included in this research were approved by…