A genome-wide MAGIC kit for recombinase-independent mosaic analysis in Drosophila

Genetic reagent (D. melanogaster)gRNA-40D2-tgFE-uHAllen et al., 2021w; gRNA-40D2-tgFE-uHVK00037Genetic reagent (D. melanogaster)gRNA-40D2-Qtg2.1-uDEHThis studyRRID:BDSC_606005w; gRNA-40D2-Qtg2.1-uDEHVK00037; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-40D4-tgFE-uHAllen et al., 2021w; gRNA-40D4-tgFE-uHVK00037Genetic reagent (D. melanogaster)gRNA-40E1-tgFE-uHAllen et al., 2021RRID:BDSC_606004w; gRNA-40E1-tgFE-uHVK00037Genetic reagent (D. melanogaster)gRNA-42A4-Qtg2.1-uDEHThis studyw; gRNA-42A4-Qtg2.1-uDEHVK00018; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-41F9-Qtg2.1-uDEHThis studyRRID:BDSC_606006w; gRNA-41F9-Qtg2.1-uDEHVK00018; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-41F11-Qtg2.1-uDEHThis studyRRID:BDSC_606007w; gRNA-41F11-Qtg2.1-uDEHVK00018; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-42A4-Qtg2.1-tDEHThis studyw; gRNA-42A4-Qtg2.1-tDEHVK00018; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-42A4-Qtg2.1-tDESThis studyRRID:BDSC_606008w; gRNA-42A4-Qtg2.1-tDESVK00018; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-80F5-Qtg2.1-tDEHThis studyRRID:BDSC_606014w; gRNA-80F5-Qtg2.1-tDEHattP2; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-80C2-Qtg2.1-tDEHThis studyRRID:BDSC_606013w; gRNA-80C2-Qtg2.1-tDEHattP2; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-80C1-Qtg2.1-tDEHThis studyRRID:BDSC_606012w; gRNA-80C1-Qtg2.1-tDEHattP2; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-81F-Qtg2.1-tDEHThis studyRRID:BDSC_606020w; gRNA-81F-Qtg2.1-tDEHVK00027; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-82A4-Qtg2.1-tDEHThis studyRRID:BDSC_606018w; gRNA-82A4-Qtg2.1-tDEHVK00027; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-82C3-Qtg2.1-tDEHThis studyRRID:BDSC_606019w; gRNA-82C3-Qtg2.1-tDEHVK00027; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-X2-Qtg2.1-tDESThis studyRRID:BDSC_606024w; gRNA-X2-Qtg2.1-tDESattP18; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-20F1-Qtg2.1-tDESThis studyRRID:BDSC_606025w; gRNA-20F1-Qtg2.1-tDESattP18; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-20F2-Qtg2.1-tDESThis studyRRID:BDSC_606026w; gRNA-20F2-Qtg2.1-tDESattP18; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-101F1a-Qtg2.1-tDESThis studyRRID:BDSC_606776w; gRNA-101F1a-Qtg2.1-tDESattP[102D]; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-101F1b-Qtg2.1-tDESThis studyRRID:BDSC_606777w; gRNA-101F1b-Qtg2.1-tDESattP[102D]; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-101F1c-Qtg2.1-tDESThis studyRRID:BDSC_606778w; gRNA-101F1c-Qtg2.1-tDESattP[102D]; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-40D2-nlsBFPAllen et al., 2021w; gRNA-40D2-nlsBFPVK00037Genetic reagent (D. melanogaster)gRNA-40D4-nlsBFPAllen et al., 2021w; gRNA-40D4-nlsBFPVK00037Genetic reagent (D. melanogaster)gRNA-40E1-nlsBFPAllen et al., 2021RRID:BDSC_606003w; gRNA-40E1-nlsBFPVK00037Genetic reagent (D. melanogaster)gRNA-40D2-tub-IFP-HAThis studyw; gRNA-40D2-tub-IFP-HAVK00037; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-42A4-tub-BFP-HAThis studyRRID:BDSC_606011w; gRNA-42A4-tub-BFP-HAVK00018; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-41F9-tub-BFP-HAThis studyRRID:BDSC_606010w; gRNA-41F9-tub-BFP-HAVK00018; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-41F11-tub-BFP-HAThis studyRRID:BDSC_606009w; gRNA-41F11-tub-BFP-HAVK00018; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-80F5-tub-BFP-HAThis studyRRID:BDSC_606017w; gRNA-80F5-tub-BFP-HAattP2; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-80C2-tub-BFP-HAThis studyRRID:BDSC_606016w; gRNA-80C2-tub-BFP-HAattP2; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-80C1-tub-BFP-HAThis studyRRID:BDSC_606015w; gRNA-80C1-tub-BFP-HAattP2; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-81F-tub-BFP-HAThis studyRRID:BDSC_606021w; gRNA-81F-tub-BFP-HAVK00027; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-82A4-tub-BFP-HAThis studyRRID:BDSC_606022w; gRNA-82A4-tub-BFP-HAVK00027; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-82C3-tub-BFP-HAThis studyRRID:BDSC_606023w; gRNA-82C3-tub-BFP-HAVK00027; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-X2-tub-BFP-HAThis studyRRID:BDSC_606028w; gRNA-X2-tub-BFP-HAattP18; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-20F1-tub-BFP-HAThis studyRRID:BDSC_606779w; gRNA-20F1-tub-BFP-HAattP18; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-20F2-tub-BFP-HAThis studyRRID:BDSC_606029w; gRNA-20F2-tub-BFP-HAattP18; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-101F1a-tub-BFP-HAThis studyRRID:BDSC_606780w; gRNA-101F1a-tub-BFP-HAattP[102D]; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-101F1b-tub-BFP-HAThis studyRRID:BDSC_606781w; gRNA-101F1b-tub-BFP-HAattP[102D]; in Materials and methodsGenetic reagent (D. melanogaster)gRNA-101F1c-tub-BFP-HAThis studyRRID:BDSC_606782w; gRNA-101F1c-tub-BFP-HAattP[102D]; in Materials and methodsGenetic reagent (D. melanogaster)pxn-Gal4Han et al., 2014Genetic reagent (D. melanogaster)UAS-CD4-tdTomHan et al., 2011RRID:BDSC_35841UAS-CD4-tdTom7M1Genetic reagent (D. melanogaster)hh-Cas9Poe et al., 2019R28E04-Cas96AGenetic reagent (D. melanogaster)zk-cas9Allen et al., 2021zk-Cas9VK00037Genetic reagent (D. melanogaster)hs-cas9Garcia-Marques et al., 2020Genetic reagent (D. melanogaster)Gal421-7Han et al., 2011Genetic reagent (D. melanogaster)UAS-MApHSHan et al., 2014UAS-MApHSVK00019Genetic reagent (D. melanogaster)RabX4-Gal4Bloomington Drosophila Stock CenterRRID:BDSC_51602Genetic reagent (D. melanogaster)R38F11-Gal4Bloomington Drosophila Stock CenterRRID:BDSC_50014R38F11-Gal4attP2Genetic reagent (D. melanogaster)ey-Cas9Ji et al., 2022ey-Cas9VK00005Genetic reagent (D. melanogaster)vas-cas9López Del Amo et al., 2022Genetic reagent (D. melanogaster)tubP(FRT.stop)Gal4 UAS-Flp UAS-mCD8::GFPKoreman et al., 2021Genetic reagent (D. melanogaster)α-Catenin-GFPBloomington Drosophila Stock CenterRRID:BDSC_58787w[*]; P{w[+mC]=UAS-alpha-Cat.T:GFP.sg}3/CyOGenetic reagent (D. melanogaster)gcm-Cas9Chen et al., 2024Genetic reagent (D. melanogaster)repo-Gal4Bloomington Drosophila Stock CenterRRID:BDSC_7415Genetic reagent (D. melanogaster)Act5C-Cas9Bloomington Drosophila Stock CenterRRID:BDSC_54590Act5C-Cas9.PGenetic reagent (D. melanogaster)vGlutSS1Bloomington Drosophila Stock CenterRRID:BDSC_91246Genetic reagent (D. melanogaster)brpd09839Bloomington Drosophila Stock CenterRRID:BDSC_85508Genetic reagent (D. melanogaster)EcRM554fsBloomington Drosophila Stock CenterRRID:BDSC_4894Genetic reagent (D. simulans)Lhr1 (D. simulans)Brideau et al., 2006Genetic reagent (D. melanogaster)Df(4)ED6380Bloomington Drosophila Stock CenterRRID:BDSC_602664Recombinant DNA reagentpAC-U63-QtgRNA2.1-ubiGal80(DE)-His2AV (plasmid)This studyin Materials and methodsRecombinant DNA reagentpAC-U63-QtgRNA2.1-tubGal80(DE)-His2AV (plasmid)This studyin Materials and methodsRecombinant DNA reagentpAC-U63-QtgRNA2.1-tubGal80(DE)-SV40 (plasmid)This studyin Materials and methodsRecombinant DNA reagentpAC-U63-QtgRNA2.1-tubBFP(HA) (plasmid)This studyin Materials and methodsRecombinant DNA reagentpAC-U63-QtgRNA2.1-tub-miRFP680-T2A-HO1(HA) (plasmid)This studyin Materials and methodsRecombinant DNA reagentpAC-U63-QtgRNA2.1–8 R (plasmid)Koreman et al., 2021RRID:Addgene 170514Recombinant DNA reagentpENTR221-tubP (plasmid)Chen et al., 2025Recombinant DNA reagentpAPIC-PHCS (plasmid)Han et al., 2011Recombinant DNA reagentpAC-U63-QtgRNA2.1-BR (plasmid)Koreman et al., 2021RRID:Addgene 170513SoftwareFijihttps://fiji.sc/RRID:SCR_002285SoftwareRhttps://www.r-project.org/RRID:SCR_001905SoftwareAdobe PhotoshopAdobeRRID:SCR_014199SoftwareAdobe IllustratorAdobeRRID:SCR_010279Chemical compoundDAPI (4′,6-Diamidino-2-Phenylindole)Life TechnologyRRID:622481:1000 dilutionAntibodyanti-Elav 7E8A10 (Rat monoclonal)Developmental Studies Hybridoma BankRRID:AB_5282181:50 dilutionAntibodyanti-Repo 8D12 (Mouse monoclonal)Developmental Studies Hybridoma BankRRID:AB_5284481:50 dilutionAntibodyanti-Brp nc82 (Mouse monoclonal)Developmental Studies Hybridoma BankRRID:AB_23148661:100 dilutionAntibodyanti-vGluT (Rabbit polyclonal)Chen et al., 20241:200 dilutionAntibodyanti-HA 12CA5 (mouse monoclonal)Sigma AldrichRoche 115838160011:100 dilutionAntibodyanti-HRP conjugated with Cy3 (goat polyclonal)Jackson ImmunoResearchRRID:AB_23389521:200 dilutionAntibodyanti-mouse secondary antibody conjugated with Cy5 (donkey polyclonal)Jackson ImmunoResearchRRID:AB_23408201:400 dilutionAntibodyanti-rabbit secondary antibody conjugated with Cy5 (donkey polyclonal)Jackson ImmunoResearchRRID:AB_23406071:400 dilutionAntibodyanti-rat secondary antibody conjugated with Cy5 (donkey polyclonal)Jackson ImmunoResearchRRID:AB_23406721:400 dilutionCommercial assay or kitT4 ligaseNew England Biolabs Inc.#M0202Commercial assay or kitNEBuilder HiFi DNA Assembly Master MixNew England Biolabs Inc.#E2621