The infamous act, who gained prominence earlier this year for leading chants of ‘death to the IDF’ at Glastonbury, made the inflammatory remarks at a gig in Amsterdam.
Frontman Bobby Vylan, real name Pascal Robinson-Foster, told the crowd: “I want to dedicate this next one to an absolute piece of s*** of a human being.
“The pronouns was/were. Because if you talk s***, you will get Banged. Rest in peace Charlie Kirk you piece of s***.”
Right wing US political figure Mr Kirk, the chief executive and co-founder of the conservative youth organisation Turning Point USA, was shot dead at an event in Utah.
Bob Vylan made the remark at a gig in Amsterdam’s Paradiso venue.
During the Amsterdam performance, Mr Robinson-Foster reportedly urged concert-goers to physically assault political opponents in the streets.
He shouted on stage: “F*** the Zionists. Go find them in the streets.”
He opened the show by asking the audience “Are there any snipers in the hall?” in what appeared to be a reference to Mr Kirk’s shooting.
One person posted online: “This guy is a disgrace to humanity.”
Another said: “He is the very definition of evil, and no one should ever buy his tickets.”
Chanan Hertzberger, the chairman of the Dutch Central Jewish council, said: “Singer Bob Vylan’s call from the stage, ‘go find them on the streets’, at Paradiso, is nothing more than a call for another pogrom in Amsterdam, like the one we saw on the night of November 7-8 last year.”
Dutch officials are being urged to intervene ahead of other performances by Bob Vylan planned next week.
Police are still investigating Bob Vylan’s Glastonbury performance.
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Dutch MPs across the political spectrum have condemned the comments. Geert Wilders, the leader of the hard-right Freedom party, accused Bob Vylan of a “call to violence and a new Jew hunt”, posting on X: “Insane that this deranged lunatic is allowed to perform in the Netherlands.”
Rob Jetten, the leader of the liberal Democrats 66 party, condemned Bob Vylan’s comments about Kirk: “You haven’t understood anything about freedom of speech and how you can disagree with each other,” he said.