{"id":330458,"date":"2026-03-05T15:08:09","date_gmt":"2026-03-05T15:08:09","guid":{"rendered":"https:\/\/www.newsbeep.com\/ie\/330458\/"},"modified":"2026-03-05T15:08:09","modified_gmt":"2026-03-05T15:08:09","slug":"enhanced-fluorescence-technique-optimises-bacterial-detection","status":"publish","type":"post","link":"https:\/\/www.newsbeep.com\/ie\/330458\/","title":{"rendered":"Enhanced fluorescence technique optimises bacterial detection"},"content":{"rendered":"

Study findings could improve fluorescence-based bacterial quantification.<\/p>\n

\"fluorescence<\/p>\n

An enhanced fluorescence-based bacterial detection<\/a> method has shown promise as a highly sensitive alternative to conventional laser-induced fluorescence (LIF) approaches.<\/p>\n

Researchers at Cairo University developed three enhanced approaches: reflection-enhanced LIF (RELIF), wavefront-enhanced LIF (WELIF), and a combined approach (WERELIF).<\/p>\n

LIF enables fluorescence to be \u201ccollected at multiple angles relative to the collimated laser beam, enabling high-resolution 2D and 3D imaging\u201d.<\/p>\n

Traditional bacterial detection methods are time-consuming and expensive, so the industry has significant need for improved alternatives, prompting the study.<\/p>\n

The team chose Pseudomonas aeruginosa (P. aeruginosa) as a model organism, due to its fluorescence properties and common association with infections and the formation of biofilms<\/a>. They assessed the bacteria without isolating or labelling its individual chromophores. Arabi et al. excited fluorescence to 405nm, reporting a peak at approximately 500nm.<\/p>\n

WERELIF was found to be the most effective detection method at low bacterial concentrations, as it \u201cdemonstrated the highest fluorescence intensity and the lowest limit of detection (LOD)\u201d, the authors explained.<\/p>\n

The method\u2019s design involved a flattop beam that redistributed energy evenly across a wider area, \u201censuring uniform illumination of fluorophores\u201d, which enhanced signal consistency and improved the reliability of integrated fluorescence intensity measurements, Arabi et al. explained.<\/p>\n

[WERELIF] merges wavefront and reflection enhancement to achieve unparalleled performance, enabling\u2026 the highest sensitivity for detecting traces of bacteria, even in highly diluted samples\u201d<\/p>\n

Critically, the technique \u201cmerges wavefront and reflection enhancement to achieve unparalleled performance\u201d, enabling \u201csuperior fluorescence retention with minimal signal attenuation, the highest sensitivity for detecting traces of bacteria, even in highly diluted samples, and enhanced spectral consistency by mitigating distortions inherent to WELIF\u201d.<\/p>\n

The enhanced methods \u201cimprove sensitivity, lower the limit of detection (LOD), and strengthen quantification accuracy\u201d for bacterial detection.<\/p>\n

The authors proposed that future investigation could \u201cfurther integrate machine learning-driven calibration\u201d to enhance sensitivity and accuracy.<\/p>\n

The paper was published in AMB Express<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"Study findings could improve fluorescence-based bacterial quantification. An enhanced fluorescence-based bacterial detection method has shown promise as a…\n","protected":false},"author":2,"featured_media":330459,"comment_status":"","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[61,60,80],"class_list":{"0":"post-330458","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-technology","8":"tag-ie","9":"tag-ireland","10":"tag-technology"},"_links":{"self":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/posts\/330458","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/comments?post=330458"}],"version-history":[{"count":0,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/posts\/330458\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/media\/330459"}],"wp:attachment":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/media?parent=330458"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/categories?post=330458"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/tags?post=330458"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}