Background and objectives<\/p>\n
Chronic pancreatitis is an inflammatory disease and is difficult to manage despite advancements in medical science. This study examined the effect of water\/ethanol extracts of\u00a0Justicia carnea\u00a0leaves on oxidative stress and glucagon expression in a mouse model of chronic pancreatitis induced by trinitrobenzenesulfonic acid (TNBS).<\/p>\n
Methods<\/p>\n
Twenty-five male Swiss albino mice were randomized and treated intrarectally with vehicle (the control group) or TNBS. Some TNBS-treated mice were treated orally with 200 mg\/kg or 400 mg\/kg\u00a0J. carnea\u00a0extracts, or with the positive control, 500 mg\/kg sulfasalazine, every other day on three occasions. Oxidative stress markers and pancreatic glucagon<\/a> expression were assessed.<\/p>\n Results<\/p>\n Compared with the healthy control mice, treatment with TNBS significantly decreased the levels of pancreatic glutathione (0.89 \u00b5mol\/g tissue vs. 7.16 \u00b5mol\/g tissue in the control) and glutathione peroxidase activity, but significantly increased the levels of \u03b1-amylase and lipase activities, lipid peroxidation, total antioxidant capacity, and nitric oxide, as well as serum C-reactive protein (P\u00a0< 0.05 for all), accompanied by severe inflammation<\/a> and reduced glucagon expression in the pancreatic tissues. The toxic effects of TNBS were significantly mitigated by treatment with\u00a0J. carnea\u00a0extracts.<\/p>\n Conclusions<\/p>\n In mice with pancreatitis initiated by TNBS, extract from\u00a0J. carnea\u00a0showed strong antioxidant and anti-inflammatory<\/a> properties. While increasing levels of antioxidant enzymes including GSH, GPx, SOD, CAT, and TAC, the therapy effectively decreased oxidative stress markers like MDA. Additionally, a noteworthy anti-inflammatory effect is indicated by the reduction in NO, lipase, and \u03b1-amylase levels. The study’s immunohistochemical and histological analyses provide evidence of\u00a0J. carnea’s\u00a0ability to effectively treat pancreatic damage instigated by TNBS.<\/p>\n Source:<\/p>\n Journal reference:<\/p>\n Enaohwo, M. T., et al. (2026). Ethanol Extracts of Justicia carnea Leaves Mitigate Pancreatic Oxidative Stress and Preserve Islet Glucagon Expression in TNBS-treated Mice. Journal of Exploratory Research in Pharmacology. DOI:\u00a010.14218\/jerp.2025.00044.\u00a0https:\/\/www.xiahepublishing.com\/2572-5505\/JERP-2025-00044<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"Background and objectives Chronic pancreatitis is an inflammatory disease and is difficult to manage despite advancements in medical…\n","protected":false},"author":2,"featured_media":269637,"comment_status":"","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[10],"tags":[9300,2684,9563,90660,19297,103,61,30608,60,205751,33772,12948,13952,10152,89,435],"class_list":["post-477079","post","type-post","status-publish","format-standard","has-post-thumbnail","category-health","tag-anti-inflammatory","tag-antioxidant","tag-chronic","tag-ethanol","tag-glucagon","tag-health","tag-ie","tag-inflammatory-disease","tag-ireland","tag-lipase","tag-mouse-model","tag-oxidative-stress","tag-pancreatitis","tag-pharmacology","tag-research","tag-stress"],"_links":{"self":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/posts\/477079","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/comments?post=477079"}],"version-history":[{"count":0,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/posts\/477079\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/media\/269637"}],"wp:attachment":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/media?parent=477079"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/categories?post=477079"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/tags?post=477079"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}