{"id":669835,"date":"2026-10-30T12:16:16","date_gmt":"2026-10-30T12:16:16","guid":{"rendered":"https:\/\/www.newsbeep.com\/ie\/669835\/"},"modified":"2026-10-30T12:16:16","modified_gmt":"2026-10-30T12:16:16","slug":"cleavage-of-xanthone-and-flavone-c-glycosidic-bonds-by-the-enzyme-complex-lagme-from-gut-lachnospiraceae","status":"publish","type":"post","link":"https:\/\/www.newsbeep.com\/ie\/669835\/","title":{"rendered":"Cleavage of xanthone and flavone C\u2013glycosidic bonds by the enzyme complex LaGME from gut Lachnospiraceae"},"content":{"rendered":"<p>Aglycones, the bioactive metabolites of C\u2013glycosides, can be obtained through the deglycosylation of naturally abundant C\u2013glycosides. However, C\u2013glycosides are more resistant to enzymatic and chemical degradation. Gut microbiota and its enzymes are known to cleave the C\u2013C glycosidic bond, but only a limited number of active strains and functional enzymes have been identified. Here we show that a new C\u2013deglycosylation gene cluster encoding the glycoside-metabolizing enzyme from Lachnospiraceae (LaGME), an enzyme complex consisting of a C\u2013glycoside oxidoreductase (LaCGO1) and a C\u2013glycoside elimination enzyme complex (LaCGE), was discovered using the DUF6379 domain-containing protein as a probe. LaGME demonstrates significant activities toward xanthone-type and flavone-type C\u2013glycosides. After investigation into heterologous expression, structural determination and catalytic mechanism, a LaGME-mediated one-pot cascade enzymatic reaction system was developed for green production of aglycones. This research provides an approach to uncover C\u2013glycoside-metabolizing enzymes in nature, as well as a preliminary basis for the sustainable production of value-added bioactive aglycones from glycosides.<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.newsbeep.com\/ie\/wp-content\/uploads\/2026\/10\/42003_2026_10896_Figa_HTML.png\" alt=\"\" loading=\"lazy\" width=\"685\" height=\"335\"\/><\/p>\n","protected":false},"excerpt":{"rendered":"Aglycones, the bioactive metabolites of C\u2013glycosides, can be obtained through the deglycosylation of naturally abundant C\u2013glycosides. However, C\u2013glycosides&hellip;\n","protected":false},"author":2,"featured_media":669836,"comment_status":"","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[33143,2368,1437,61,60,2137,2746,82],"class_list":["post-669835","post","type-post","status-publish","format-standard","has-post-thumbnail","category-science","tag-biocatalysis","tag-biotechnology","tag-general","tag-ie","tag-ireland","tag-life-sciences","tag-microbiology","tag-science"],"_links":{"self":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/posts\/669835","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/comments?post=669835"}],"version-history":[{"count":0,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/posts\/669835\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/media\/669836"}],"wp:attachment":[{"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/media?parent=669835"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/categories?post=669835"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.newsbeep.com\/ie\/wp-json\/wp\/v2\/tags?post=669835"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}