{"id":212721,"date":"2025-12-31T06:59:11","date_gmt":"2025-12-31T06:59:11","guid":{"rendered":"https:\/\/www.newsbeep.com\/il\/212721\/"},"modified":"2025-12-31T06:59:11","modified_gmt":"2025-12-31T06:59:11","slug":"endonuclease-v-activated-pyrococcus-furiosus-argonaute-for-the-detection-of-food-contaminated-bacteria","status":"publish","type":"post","link":"https:\/\/www.newsbeep.com\/il\/212721\/","title":{"rendered":"Endonuclease V activated Pyrococcus furiosus Argonaute for the detection of food contaminated bacteria"},"content":{"rendered":"<p class=\"c-article-references__text\" id=\"ref-CR1\">Banada, P. P. et al. Label-free detection of multiple bacterial pathogens using light-scattering sensor. Biosens. Bioelectron. 24, 1685\u20131692 (2009).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 1\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Label-free%20detection%20of%20multiple%20bacterial%20pathogens%20using%20light-scattering%20sensor&amp;journal=Biosens.%20Bioelectron.&amp;volume=24&amp;pages=1685-1692&amp;publication_year=2009&amp;author=Banada%2CPP\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR2\">Gao, B. et al. Urease-induced metallization of gold nanorods for the sensitive detection of Salmonella enterica Choleraesuis through colorimetric ELISA. J. Dairy Sci. 102, 1997\u20132007 (2019).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 2\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Urease-induced%20metallization%20of%20gold%20nanorods%20for%20the%20sensitive%20detection%20of%20Salmonella%20enterica%20Choleraesuis%20through%20colorimetric%20ELISA&amp;journal=J.%20Dairy%20Sci.&amp;volume=102&amp;pages=1997-2007&amp;publication_year=2019&amp;author=Gao%2CB\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR3\">Xue, L., Zheng, L., Zhang, H., Jin, X. &amp; Lin, J. An ultrasensitive fluorescent biosensor using high gradient magnetic separation and quantum dots for fast detection of foodborne pathogenic bacteria. Sens. Actuators B Chem. 265, 318\u2013325 (2018).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 3\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=An%20ultrasensitive%20fluorescent%20biosensor%20using%20high%20gradient%20magnetic%20separation%20and%20quantum%20dots%20for%20fast%20detection%20of%20foodborne%20pathogenic%20bacteria&amp;journal=Sens.%20Actuators%20B%20Chem.&amp;volume=265&amp;pages=318-325&amp;publication_year=2018&amp;author=Xue%2CL&amp;author=Zheng%2CL&amp;author=Zhang%2CH&amp;author=Jin%2CX&amp;author=Lin%2CJ\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR4\">Wu, M. Y.-C. et al. Point-of-care detection devices for food safety monitoring: proactive disease prevention. Trends Biotechnol. 35, 288\u2013300 (2017).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 4\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Point-of-care%20detection%20devices%20for%20food%20safety%20monitoring%3A%20proactive%20disease%20prevention&amp;journal=Trends%20Biotechnol.&amp;volume=35&amp;pages=288-300&amp;publication_year=2017&amp;author=Wu%2CMY-C\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR5\">Lee, H. &amp; Yoon, Y. Etiological agents implicated in foodborne illness World Wide. Food Sci. Anim. Resour. 41, 1\u20137 (2021).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 5\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Etiological%20agents%20implicated%20in%20foodborne%20illness%20World%20Wide&amp;journal=Food%20Sci.%20Anim.%20Resour.&amp;volume=41&amp;pages=1-7&amp;publication_year=2021&amp;author=Lee%2CH&amp;author=Yoon%2CY\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR6\">Li, Y. et al. Argonaute-triggered visual and rebuilding-free foodborne pathogenic bacteria detection. J. Hazard. Mater. 454, 131485 (2023).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 6\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Argonaute-triggered%20visual%20and%20rebuilding-free%20foodborne%20pathogenic%20bacteria%20detection&amp;journal=J.%20Hazard.%20Mater.&amp;volume=454&amp;publication_year=2023&amp;author=Li%2CY\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR7\">Wang, Z., Cheng, X., Ma, A., Jiang, F. &amp; Chen, Y. Multiplexed food-borne pathogen detection using an argonaute-mediated digital sensor based on a magnetic-bead-assisted imaging transcoding system. Nat. Food 6, 170\u2013181 (2025).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 7\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Multiplexed%20food-borne%20pathogen%20detection%20using%20an%20argonaute-mediated%20digital%20sensor%20based%20on%20a%20magnetic-bead-assisted%20imaging%20transcoding%20system&amp;journal=Nat.%20Food&amp;volume=6&amp;pages=170-181&amp;publication_year=2025&amp;author=Wang%2CZ&amp;author=Cheng%2CX&amp;author=Ma%2CA&amp;author=Jiang%2CF&amp;author=Chen%2CY\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR8\">Veld, J. H. J. H. in\u2019t. Microbial and biochemical spoilage of foods: an overview. Int. J. Food Microbiol. 33, 1\u201318 (1996).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 8\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=in%E2%80%99t.%20Microbial%20and%20biochemical%20spoilage%20of%20foods%3A%20an%20overview&amp;journal=Int.%20J.%20Food%20Microbiol.&amp;volume=33&amp;pages=1-18&amp;publication_year=1996&amp;author=Veld%2CJHJH\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR9\">Martinovi\u0107, T., Andjelkovi\u0107, U., Gajdo\u0161ik, M. \u0160, Re\u0161etar, D. &amp; Josi\u0107, D. Foodborne pathogens and their toxins. J. Proteom. 147, 226\u2013235 (2016).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 9\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Foodborne%20pathogens%20and%20their%20toxins&amp;journal=J.%20Proteom.&amp;volume=147&amp;pages=226-235&amp;publication_year=2016&amp;author=Martinovi%C4%87%2CT&amp;author=Andjelkovi%C4%87%2CU&amp;author=Gajdo%C5%A1ik%2CM%C5%A0&amp;author=Re%C5%A1etar%2CD&amp;author=Josi%C4%87%2CD\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR10\">Zamfir, L.-G. et al. Assessment of biogenic amines produced by microorganisms as food spoilage indicators by sensitive detection using portable opto-electrochemical tools based on biosensors. Food Control 172, 111161 (2025).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 10\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Assessment%20of%20biogenic%20amines%20produced%20by%20microorganisms%20as%20food%20spoilage%20indicators%20by%20sensitive%20detection%20using%20portable%20opto-electrochemical%20tools%20based%20on%20biosensors&amp;journal=Food%20Control&amp;volume=172&amp;publication_year=2025&amp;author=Zamfir%2CL-G\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR11\">Song, J. et al. Smartphone-Based SARS-CoV-2 and variants detection system using colorimetric DNAzyme reaction triggered by Loop-Mediated Isothermal Amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR). ACS Nano 16, 11300\u201311314 (2022).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 11\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Smartphone-Based%20SARS-CoV-2%20and%20variants%20detection%20system%20using%20colorimetric%20DNAzyme%20reaction%20triggered%20by%20Loop-Mediated%20Isothermal%20Amplification%20%28LAMP%29%20with%20clustered%20regularly%20interspaced%20short%20palindromic%20repeats%20%28CRISPR%29&amp;journal=ACS%20Nano&amp;volume=16&amp;pages=11300-11314&amp;publication_year=2022&amp;author=Song%2CJ\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR12\">Guo, B. et al. The detection of Salmonella in food based on PCR combined with Pyrococcus furiosus Argonaute. Microchem. J. 206, 111311 (2024).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 12\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=The%20detection%20of%20Salmonella%20in%20food%20based%20on%20PCR%20combined%20with%20Pyrococcus%20furiosus%20Argonaute&amp;journal=Microchem.%20J.&amp;volume=206&amp;publication_year=2024&amp;author=Guo%2CB\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR13\">Swarts, D. C. et al. Autonomous generation and loading of DNA guides by bacterial argonaute. Mol. Cell 65, 985\u2013998.e6 (2017).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 13\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Autonomous%20generation%20and%20loading%20of%20DNA%20guides%20by%20bacterial%20argonaute&amp;journal=Mol.%20Cell&amp;volume=65&amp;pages=985-998.e6&amp;publication_year=2017&amp;author=Swarts%2CDC\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR14\">Xun, G. et al. Argonaute with stepwise endonuclease activity promotes specific and multiplex nucleic acid detection. Bioresour. Bioprocess. 8, 46 (2021).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 14\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Argonaute%20with%20stepwise%20endonuclease%20activity%20promotes%20specific%20and%20multiplex%20nucleic%20acid%20detection&amp;journal=Bioresour.%20Bioprocess.&amp;volume=8&amp;publication_year=2021&amp;author=Xun%2CG\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR15\">Lin, L. et al. Recombinase polymerase amplification combined with Pyrococcus furiosus Argonaute for fast Salmonella spp. testing in food safety. Int. J. Food Microbiol. 417, 110697 (2024).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 15\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Recombinase%20polymerase%20amplification%20combined%20with%20Pyrococcus%20furiosus%20Argonaute%20for%20fast%20Salmonella%20spp.%20testing%20in%20food%20safety&amp;journal=Int.%20J.%20Food%20Microbiol.&amp;volume=417&amp;publication_year=2024&amp;author=Lin%2CL\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR16\">Liu, Y. et al. Development and application of a novel recombinase polymerase amplification Pyrococcus furiosus argonaute system for rapid detection of goose parvovirus. Poult. Sci. 103, 104141 (2024).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 16\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Development%20and%20application%20of%20a%20novel%20recombinase%20polymerase%20amplification%20Pyrococcus%20furiosus%20argonaute%20system%20for%20rapid%20detection%20of%20goose%20parvovirus&amp;journal=Poult.%20Sci.&amp;volume=103&amp;publication_year=2024&amp;author=Liu%2CY\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR17\">Yu, Z. et al. LAMP combined with Pyrococcus furiosus Argonaute for the ultrasensitive and highly specific point-of-care test platform for Listeria monocytogenes detection. LWT 207, 116640 (2024).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 17\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=LAMP%20combined%20with%20Pyrococcus%20furiosus%20Argonaute%20for%20the%20ultrasensitive%20and%20highly%20specific%20point-of-care%20test%20platform%20for%20Listeria%20monocytogenes%20detection&amp;journal=LWT&amp;volume=207&amp;publication_year=2024&amp;author=Yu%2CZ\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR18\">Swarts, D. C. et al. Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that targets cognate DNA. Nucleic Acids Res. 43, 5120\u20135129 (2015).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 18\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Argonaute%20of%20the%20archaeon%20Pyrococcus%20furiosus%20is%20a%20DNA-guided%20nuclease%20that%20targets%20cognate%20DNA&amp;journal=Nucleic%20Acids%20Res.&amp;volume=43&amp;pages=5120-5129&amp;publication_year=2015&amp;author=Swarts%2CDC\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR19\">Dizdaroglu, M., Laval, J. &amp; Boiteux, S. Substrate specificity of the Escherichia coli endonuclease III: excision of thymine- and cytosine-derived lesions in DNA produced by radiation-generated free radicals. Biochemistry 32, 12105\u201312111 (1993).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 19\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Substrate%20specificity%20of%20the%20Escherichia%20coli%20endonuclease%20III%3A%20excision%20of%20thymine-%20and%20cytosine-derived%20lesions%20in%20DNA%20produced%20by%20radiation-generated%20free%20radicals&amp;journal=Biochemistry&amp;volume=32&amp;pages=12105-12111&amp;publication_year=1993&amp;author=Dizdaroglu%2CM&amp;author=Laval%2CJ&amp;author=Boiteux%2CS\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR20\">Yao, M. &amp; Kow, Y. W. Strand-specific cleavage of mismatch-containing DNA by deoxyinosine 3\u2019-endonuclease from Escherichia coli. J. Biol. Chem. 269, 31390\u201331396 (1994).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 20\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Strand-specific%20cleavage%20of%20mismatch-containing%20DNA%20by%20deoxyinosine%203%E2%80%99-endonuclease%20from%20Escherichia%20coli&amp;journal=J.%20Biol.%20Chem.&amp;volume=269&amp;pages=31390-31396&amp;publication_year=1994&amp;author=Yao%2CM&amp;author=Kow%2CYW\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR21\">Yu, Z. et al. LAMP assay coupled with a pyrococcus furiosus argonaute system for the rapid detection of porcine epidemic diarrhea virus. ACS Synth. Biol. <a href=\"https:\/\/doi.org\/10.1021\/acssynbio.4c00446\" data-track=\"click_references\" data-track-action=\"external reference\" data-track-value=\"external reference\" data-track-label=\"10.1021\/acssynbio.4c00446\" rel=\"nofollow noopener\" target=\"_blank\">https:\/\/doi.org\/10.1021\/acssynbio.4c00446<\/a> (2025).<\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR22\">Pang, F. et al. A handheld isothermal fluorescence detector for duplex visualization of aquatic pathogens via enhanced one-pot LAMP-PfAgo assay. Biosens. Bioelectron. 254, 116187 (2024).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 22\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=A%20handheld%20isothermal%20fluorescence%20detector%20for%20duplex%20visualization%20of%20aquatic%20pathogens%20via%20enhanced%20one-pot%20LAMP-PfAgo%20assay&amp;journal=Biosens.%20Bioelectron.&amp;volume=254&amp;publication_year=2024&amp;author=Pang%2CF\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR23\">Shi, Y., Tan, Z., Wu, D., Wu, Y. &amp; Li, G. Pyrococcus furiosus argonaute based Alicyclobacillus acidoterrestrsis detection in fruit juice. Food Microbiol. 120, 104475 (2024).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 23\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Pyrococcus%20furiosus%20argonaute%20based%20Alicyclobacillus%20acidoterrestrsis%20detection%20in%20fruit%20juice&amp;journal=Food%20Microbiol.&amp;volume=120&amp;publication_year=2024&amp;author=Shi%2CY&amp;author=Tan%2CZ&amp;author=Wu%2CD&amp;author=Wu%2CY&amp;author=Li%2CG\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR24\">Shi, Y. et al. Enzyme-assisted endogenous guide DNA generation-mediated pyrococcus furiosus argonaute for alicyclobacillus acidoterrestris detection. J. Agric. Food Chem. 72, 1354\u20131360 (2024).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 24\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Enzyme-assisted%20endogenous%20guide%20DNA%20generation-mediated%20pyrococcus%20furiosus%20argonaute%20for%20alicyclobacillus%20acidoterrestris%20detection&amp;journal=J.%20Agric.%20Food%20Chem.&amp;volume=72&amp;pages=1354-1360&amp;publication_year=2024&amp;author=Shi%2CY\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR25\">Cai, M. et al. Detection of Escherichia coli O157:H7 in food based on magnetic separation and hybridization chain reaction. Food Control 177, 111446 (2025).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 25\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Detection%20of%20Escherichia%20coli%20O157%3AH7%20in%20food%20based%20on%20magnetic%20separation%20and%20hybridization%20chain%20reaction&amp;journal=Food%20Control&amp;volume=177&amp;publication_year=2025&amp;author=Cai%2CM\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR26\">Mondal, B., Bhavanashri, N., Ramlal, S. &amp; Kingston, J. Colorimetric DNAzyme biosensor for convenience detection of enterotoxin B Harboring Staphylococcus aureus from food samples. J. Agric. Food Chem. 66, 1516\u20131522 (2018).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 26\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Colorimetric%20DNAzyme%20biosensor%20for%20convenience%20detection%20of%20enterotoxin%20B%20Harboring%20Staphylococcus%20aureus%20from%20food%20samples&amp;journal=J.%20Agric.%20Food%20Chem.&amp;volume=66&amp;pages=1516-1522&amp;publication_year=2018&amp;author=Mondal%2CB&amp;author=Bhavanashri%2CN&amp;author=Ramlal%2CS&amp;author=Kingston%2CJ\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR27\">Li, G. et al. Rapid and ultrasensitive detection of Staphylococcus aureus by a one-pot system integrating pyrococcus furiosus argonaute with loop-mediated isothermal amplification. J. Microbiol. Biotechnol. 35, e2504034 (2025).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 27\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Rapid%20and%20ultrasensitive%20detection%20of%20Staphylococcus%20aureus%20by%20a%20one-pot%20system%20integrating%20pyrococcus%20furiosus%20argonaute%20with%20loop-mediated%20isothermal%20amplification&amp;journal=J.%20Microbiol.%20Biotechnol.&amp;volume=35&amp;publication_year=2025&amp;author=Li%2CG\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR28\">Lv, X. et al. Quantitative detection of trace VBNC Cronobacter sakazakii by immunomagnetic separation in combination with PMAxx-ddPCR in dairy products. Food Microbiol. 99, 103831 (2021).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 28\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Quantitative%20detection%20of%20trace%20VBNC%20Cronobacter%20sakazakii%20by%20immunomagnetic%20separation%20in%20combination%20with%20PMAxx-ddPCR%20in%20dairy%20products&amp;journal=Food%20Microbiol.&amp;volume=99&amp;publication_year=2021&amp;author=Lv%2CX\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n<p class=\"c-article-references__text\" id=\"ref-CR29\">Wang, P., Chen, Q., Wang, Y., Sun, X. &amp; Liu, Z. Development of a visual assay for detection of viable cronobacter sakazakii using RT-PSR and hydroxynaphthol blue indicator. Biology 14, 383 (2025).<\/p>\n<p class=\"c-article-references__links u-hide-print\"><a data-track=\"click_references\" data-track-action=\"google scholar reference\" data-track-value=\"google scholar reference\" data-track-label=\"link\" data-track-item_id=\"link\" rel=\"nofollow noopener\" aria-label=\"Google Scholar reference 29\" href=\"http:\/\/scholar.google.com\/scholar_lookup?&amp;title=Development%20of%20a%20visual%20assay%20for%20detection%20of%20viable%20cronobacter%20sakazakii%20using%20RT-PSR%20and%20hydroxynaphthol%20blue%20indicator&amp;journal=Biology&amp;volume=14&amp;publication_year=2025&amp;author=Wang%2CP&amp;author=Chen%2CQ&amp;author=Wang%2CY&amp;author=Sun%2CX&amp;author=Liu%2CZ\" target=\"_blank\"><br \/>\n                    Google Scholar<\/a>\u00a0\n                <\/p>\n","protected":false},"excerpt":{"rendered":"Banada, P. P. et al. Label-free detection of multiple bacterial pathogens using light-scattering sensor. Biosens. Bioelectron. 24, 1685\u20131692&hellip;\n","protected":false},"author":2,"featured_media":212722,"comment_status":"","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[4679,304,165,83912,118360,2806,3181,85,46,3488,8435,543,141],"class_list":{"0":"post-212721","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-science","8":"tag-biological-techniques","9":"tag-biotechnology","10":"tag-cancer","11":"tag-chemistry-food-science","12":"tag-food-microbiology","13":"tag-food-science","14":"tag-general","15":"tag-il","16":"tag-israel","17":"tag-microbiology","18":"tag-molecular-biology","19":"tag-nutrition","20":"tag-science"},"_links":{"self":[{"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/posts\/212721","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/comments?post=212721"}],"version-history":[{"count":0,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/posts\/212721\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/media\/212722"}],"wp:attachment":[{"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/media?parent=212721"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/categories?post=212721"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/tags?post=212721"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}