Sino Biological, Inc. has announced that its gene synthesis and cell-free protein expression workflow was used in a recent study by Tencent AI for Life Sciences Lab published in Nature Communications, enabling rapid validation of AI-designed proteins with improved activity, stability, and multifunctionality.<\/p>\n
Bridging AI protein design and experimental validation<\/p>\n
Artificial intelligence has accelerated protein amino acid sequence design, however, translating these computational designs into functional proteins remains a key challenge in protein engineering. Protein activity, stability, folding, and expression are influenced by complex structural and biochemical factors, often causing discrepancies between in silico predictions and experiments.<\/p>\n
To address this gap, the study introduced an Ontology Reinforcement Iteration (ORI) framework, integrating protein ontology with reinforcement learning from wet-lab feedback. Experimental data, including protein expression levels and functional activity, were continuously fed back into the model enabling iterative optimization of protein sequences and improved design accuracy.<\/p>\n
Cell-Free Protein Synthesis accelerates the AI design loop<\/p>\n
The researchers subsequently utilized Sino Biological\u2019s XPressMAX\u2122 Cell-Free Protein Synthesis Kit to enable rapid protein expression and functional screening. Protein-coding sequences cloned into the kit\u2019s expression vector and added to the proprietary cell-free reaction system supported rapid design\u2013build\u2013test cycles.<\/p>\n
Using this workflow, the team engineered a lysozyme with over 100-fold higher activity than the natural enzyme, developed a thermostable chitinase retaining activity at 85 \u00b0C, and expressed bifunctional enzymes with improved performance compared with naturally occurring multifunctional enzymes.<\/p>\n
XPressMAX\u2122 Cell-Free Protein Synthesis\u00a0Kit
\nKey features include:<\/p>\n
Ultra-fast synthesis: proteins expressed in as little as 3 hours
\nHigh screening efficiency: optimized for VHH, scFv, and miniproteins
\nFlexible template support: compatible with plasmid and linear DNA templates
\nDisulfide bond friendly: enables expression of complex disulfide-bonded proteins without additional enhancers
\nCost-effective performance: reduced operational time and cost
\nScalable supply: suitable for high-throughput and industrial applications<\/p>\n","protected":false},"excerpt":{"rendered":"Sino Biological, Inc. has announced that its gene synthesis and cell-free protein expression workflow was used in a…\n","protected":false},"author":2,"featured_media":19195,"comment_status":"","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[14141,343,1353,5352,85,46,5198,11814,43409,125],"class_list":["post-465713","post","type-post","status-publish","format-standard","has-post-thumbnail","category-technology","tag-amino-acid","tag-artificial-intelligence","tag-cell","tag-gene","tag-il","tag-israel","tag-protein","tag-protein-expression","tag-protein-synthesis","tag-technology"],"_links":{"self":[{"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/posts\/465713","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/comments?post=465713"}],"version-history":[{"count":0,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/posts\/465713\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/media\/19195"}],"wp:attachment":[{"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/media?parent=465713"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/categories?post=465713"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.newsbeep.com\/il\/wp-json\/wp\/v2\/tags?post=465713"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}