The road and on-ramp were reopened just after 7.30am.
Fire and Emergency incident controller Mike Dombroski said three pumps, a command unit and a water tanker were at the scene.
“We had a hazmat unit in attendance earlier this morning who helped to contain the spill.
“We are now waiting for the fuel tanker company to decant the remaining fuel from the truck before roading contractors can arrive to assist with further clean-up using roading sand.”
Aotea Quay is a key route into central Wellington from the north, and commuter traffic into the central city and access to Interislander ferries would be affected.
A spokesperson from Interislander said the crash did impact access to the terminal, but the delays are minimal.
“Passengers arriving on this morning’s Kaitaki sailing from Picton were able to disembark as usual.
“We contacted passengers booked on the 8.45am Wellington to Picton sailing directly earlier this morning to warn them of possible traffic delays and diversions, and that the sailing might be slightly delayed as a result.
“Almost all passengers for this sailing have now checked in, and we expect to depart by 9am.”
Motorists were being told to avoid the area and to expect delays.