Yang, Y. et al. Derivation of pluripotent stem cells with in vivo embryonic and extraembryonic potency. Cell 169, 243–257 (2017).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Yang, J. et al. Establishment of mouse expanded potential stem cells. Nature 550, 393 (2017).

Article 
ADS 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Shen, H. et al. Mouse totipotent stem cells captured and maintained through spliceosomal repression. Cell 184, 2843–2859 (2021).

Article 
PubMed 
CAS 

Google Scholar
 

Yang, M. et al. Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 29, 400–418 (2022).

Article 
PubMed 
CAS 

Google Scholar
 

Xu, Y. et al. Derivation of totipotent-like stem cells with blastocyst-like structure forming potential. Cell Res. 32, 513–529 (2022).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Li, R. et al. Generation of blastocyst-like structures from mouse embryonic and adult cell cultures. Cell 179, 687–702(2019).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Sozen, B. et al. Self-organization of mouse stem cells into an extended potential blastoid. Dev. Cell 51, 698–712 (2019).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Zhang, P. et al. Highly efficient generation of blastocyst-like structures from spliceosomes-repressed mouse totipotent blastomere-like cells. Sci. China Life Sci. 66, 423–435 (2023).

Article 
ADS 
PubMed 
CAS 

Google Scholar
 

Luo, Y. et al. Exploring the impacts of senescence on implantation and early embryonic development using totipotent cell-derived blastoids. J. Adv. Res. 68, 115–129 (2025).

Article 
PubMed 
CAS 

Google Scholar
 

Peng, B. et al. Mouse totipotent blastomere-like cells model embryogenesis from zygotic genome activation to post implantation. Cell Stem Cell 32, 391–408 (2025).

Article 
PubMed 
CAS 

Google Scholar
 

Ren, Y. et al. A continuous totipotent-like cell-based embryo model recapitulates mouse embryogenesis from zygotic genome activation to gastrulation. Nat. Cell Biol. 27, 1993–2006 (2025).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Li, H. et al. A complete model of mouse embryogenesis through organogenesis enabled by chemically induced embryo founder cells. Cell 188, 5912–5930 (2025).

Article 
PubMed 
CAS 

Google Scholar
 

Yilmaz, A. et al. Transgene-free generation of mouse post-gastrulation whole embryo models solely from naive ESCs and iPSCs. Cell Stem Cell 32, 1545–1562 (2025).

Article 
PubMed 
CAS 

Google Scholar
 

Liu, K. et al. Modeling post-gastrula development via bidirectional pluripotent stem cells. Cell Res. 35, 954–969 (2025).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Hu, Y. et al. Induction of mouse totipotent stem cells by a defined chemical cocktail. Nature 617, 792–797 (2023).

Article 
ADS 
PubMed 
CAS 

Google Scholar
 

Tarkowski, A. K. Experiments on the development of isolated blastomeres of mouse egg. Nature 184, 1286–1287 (1959).

Article 
ADS 
PubMed 
CAS 

Google Scholar
 

Zhu, M. & Zernicka-Goetz, M. Principles of self-organization of the mammalian embryo. Cell 183, 1467–1478 (2020).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Rivron, N. C. et al. Blastocyst-like structures generated solely from stem cells. Nature 557, 106–111 (2018).

Article 
ADS 
PubMed 
CAS 

Google Scholar
 

Harrison, S. E. et al. Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro. Science 356, eaal1810 (2017).

Article 
PubMed 

Google Scholar
 

Sozen, B. et al. Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures. Nat. Cell Biol. 20, 979–989 (2018).

Article 
PubMed 
CAS 

Google Scholar
 

Amadei, G. et al. Inducible stem-cell-derived embryos capture mouse morphogenetic events in vitro. Dev. Cell 56, 366–382 (2021).

Article 
PubMed 
CAS 

Google Scholar
 

Tarazi, S. et al. Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs. Cell 185, 3290–3306 (2022).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Amadei, G. et al. Embryo model completes gastrulation to neurulation and organogenesis. Nature 610, 143–153 (2022).

Article 
ADS 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Solter, D. From teratocarcinomas to embryonic stem cells and beyond: a history of embryonic stem cell research. Nat. Rev. Genet. 7, 319–327 (2006).

Article 
PubMed 
CAS 

Google Scholar
 

Posfai, E. et al. Evaluating totipotency using criteria of increasing stringency. Nat. Cell Biol. 23, 49–60 (2021).

Article 
PubMed 
CAS 

Google Scholar
 

Macfarlan, T. S. et al. Embryonic stem cell potency fluctuates with endogenous retrovirus activity. Nature 487, 57–63 (2012).

Article 
ADS 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Ishiuchi, T. et al. Early embryonic-like cells are induced by downregulating replication-dependent chromatin assembly. Nat. Struct. Mol. Biol. 22, 662–671 (2015).

Article 
PubMed 
CAS 

Google Scholar
 

Zhang, Y. et al. Epidermal growth factor induces a trophectoderm lineage transcriptome resembling that of human embryos during reconstruction of blastoids from extended pluripotent stem cells. Cell Prolif. 55, e13317 (2022).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Seong, J. et al. Epiblast inducers capture mouse trophectoderm stem cells in vitro and pattern blastoids for implantation in utero. Cell Stem Cell 29, 1102–1118 (2022).

Article 
MathSciNet 
PubMed 
CAS 

Google Scholar
 

Zenker, J. et al. Expanding actin rings zipper the mouse embryo for blastocyst formation. Cell 173, 776–791 (2018).

Article 
PubMed 
CAS 

Google Scholar
 

Nichols, J. & Smith, A. Naive and primed pluripotent states. Cell Stem Cell 4, 487–492 (2009).

Article 
PubMed 
CAS 

Google Scholar
 

Yanes, O. et al. Metabolic oxidation regulates embryonic stem cell differentiation. Nat. Chem. Biol. 6, 411–417 (2010).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Pijuan-Sala, B. et al. A single-cell molecular map of mouse gastrulation and early organogenesis. Nature 566, 490–495 (2019).

Article 
ADS 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Aguilera-Castrejon, A. et al. Ex utero mouse embryogenesis from pre-gastrulation to late organogenesis. Nature 593, 119–124 (2021).

Article 
ADS 
PubMed 
CAS 

Google Scholar
 

Richardson, B. & Lehmann, R. Mechanisms guiding primordial germ cell migration: strategies from different organisms. Nat. Rev. Mol. Cell Biol. 11, 37–49 (2010).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Ohta, H. et al. In vitro expansion of mouse primordial germ cell-like cells recapitulates an epigenetic blank slate. EMBO J. 36, 1888–1907 (2017).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Büttner, M., Miao, Z., Wolf, F. A., Teichmann, S. A. & Theis, F. J. A test metric for assessing single-cell RNA-seq batch correction. Nat. Methods 16, 43–49 (2019).

Article 
PubMed 

Google Scholar
 

Tran, H. T. N. et al. A benchmark of batch-effect correction methods for single-cell RNA sequencing data. Genome Biol. 21, 12 (2020).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Zhang, L. et al. Single-cell analyses inform mechanisms of myeloid-targeted therapies in colon cancer. Cell 181, 442–459 (2020).

Article 
ADS 
PubMed 
CAS 

Google Scholar
 

Li, J. et al. Accurate annotation of accessible chromatin in mouse and human primordial germ cells. Cell Res. 28, 1077–1089 (2018).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Canu, G. & Ruhrberg, C. First blood: the endothelial origins of hematopoietic progenitors. Angiogenesis 24, 199–211 (2021).

Article 
PubMed 
PubMed Central 

Google Scholar
 

Capellera-Garcia, S. et al. Defining the minimal factors required for erythropoiesis through direct lineage conversion. Cell Rep. 15, 2550–2562 (2016).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Dang, D. et al. Computational approach to identifying universal macrophage biomarkers. Front. Physiol. 11, 275 (2020).

Article 
PubMed 
PubMed Central 

Google Scholar
 

Epelman, S., Lavine, K. J. & Randolph, G. J. Origin and functions of tissue macrophages. Immunity 41, 21–35 (2014).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Meilhac, S. M. & Buckingham, M. E. The deployment of cell lineages that form the mammalian heart. Nat. Rev. Cardiol. 15, 705–724 (2018).

Article 
PubMed 

Google Scholar
 

Meilhac, S. M. et al. Cardiac cell lineages that form the heart. Cold Spring Harb. Perspect. Med. 4, a013888 (2014).

Article 
PubMed 
PubMed Central 

Google Scholar
 

Hirschy, A. et al. Establishment of cardiac cytoarchitecture in the developing mouse heart. Dev. Biol. 289, 430–441 (2006).

Article 
PubMed 
CAS 

Google Scholar
 

Epelman, S. et al. Embryonic and adult-derived resident cardiac macrophages are maintained through distinct mechanisms at steady state and during inflammation. Immunity 40, 91–104 (2014).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Stary, M. et al. Parietal endoderm secreted SPARC promotes early cardiomyogenesis in vitro. Exp. Cell. Res. 310, 331–343 (2005).

Article 
ADS 
PubMed 
CAS 

Google Scholar
 

Nascone, N. & Mercola, M. Endoderm and cardiogenesis: new insights. Trends Cardiovasc. Med. 6, 211–216 (1996).

Article 
PubMed 
CAS 

Google Scholar
 

Ohinata, Y. et al. A comprehensive, non-invasive visualization of primordial germ cell development in mice by the Blimp1-mVenus and stella-ECFP double transgenic reporter. Reproduction 136, 503–514 (2008).

Article 
PubMed 
CAS 

Google Scholar
 

Zhou, Q. et al. Complete meiosis from embryonic stem cell-derived germ cells in vitro. Cell Stem Cell 18, 330–340 (2016).

Article 
PubMed 
CAS 

Google Scholar
 

Garcia, M. D. et al. Preparation of rat serum for culturing mouse embryos. Cold Spring Harb. Protoc. 2011, prot5593 (2011).

Article 

Google Scholar
 

Takahashi, M. et al. Preparation of rat serum suitable for mammalian whole embryo culture. J. Vis. Exp. 90, e51969 (2014).


Google Scholar
 

Nosaka, Y. et al. Generation of germinal-vesicle oocytes from mouse embryonic stem cells under an ovarian soma-free condition. Dev. Cell 60, 2976–2994 (2025).

Article 
PubMed 
CAS 

Google Scholar
 

Deng, Q. et al. Single-cell RNA-seq reveals dynamic, random monoallelic gene expression in mammalian cells. Science 343, 193–197 (2014).

Article 
ADS 
PubMed 
CAS 

Google Scholar
 

Qiu, C. et al. Systematic reconstruction of cellular trajectories across mouse embryogenesis. Nat. Genet. 54, 328–341 (2022).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Mohammed, H. et al. Single-cell landscape of transcriptional heterogeneity and cell fate decisions during mouse early gastrulation. Cell Rep. 20, 1215–1228 (2017).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Cheng, S. et al. Single-cell RNA-seq reveals cellular heterogeneity of pluripotency transition and X chromosome dynamics during early mouse development. Cell Rep. 26, 2593–2607 (2019).

Article 
PubMed 
CAS 

Google Scholar
 

Chan, M. M. et al. Molecular recording of mammalian embryogenesis. Nature 570, 77–82 (2019).

Article 
ADS 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Stuart, T. et al. Comprehensive integration of single-cell data. Cell 177, 1888–1902 (2019).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Korsunsky, I. et al. Fast, sensitive and accurate integration of single-cell data with Harmony. Nat. Methods 16, 1289–1296 (2019).

Article 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Young, M. D. et al. Single-cell transcriptomes from human kidneys reveal the cellular identity of renal tumors. Science 361, 594–599 (2018).

Article 
ADS 
PubMed 
PubMed Central 
CAS 

Google Scholar
 

Sala, L. et al. MUSCLEMOTION: a versatile open software tool to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo. Circ. Res. 122, e5–e16 (2018).

Article 
PubMed 
CAS 

Google Scholar
 

van Meer, B. J. et al. Quantification of muscle contraction in vitro and in vivo using MUSCLEMOTION software: from stem cell-derived cardiomyocytes to zebrafish and human hearts. Curr. Protoc. Hum. Genet. 99, e67 (2018).

Article 
PubMed 

Google Scholar