Earlier this year, Smallbrook Road was closed via a Traffic Regulatory Order (TRO) to protect adult toads during the migratory season.
During this period, Warminster Toad Patrol volunteers took nightly shifts to observe the animals and keep them safe.
Ms James said: “We did so much patrolling in Spring to save the toadlets’ parents and get them to their mating ponds. And now, they’re being annihilated.
“There are very few motorists using Smallbrook Road, but it takes just a few cars to cause a lot of toad deaths.
“There is an alternative route through the main street, it would be great if people could use it.”