In the same group, League Two side Notts County inflicted a fourth successive defeat in all competitions on Barnsley, winning 2-1 at Meadow Lane.
Jayden Luker scored in the ninth minute and although Davis Keillor-Dunn brought the Tykes level, they then had Jonathan Bland sent off, and Tyrese Hall won it for the Magpies in the 89th-minute.
West Ham Under-21s went down to 10 men at Reading when Ezra Mayers was shown a red card after Callum Marshall had put them in front.
And the Royals made the most of their numerical advantage as Jacob Borgnis, Mark O’Mahony and Jeremiah Okine-Peters gave them a 3-1 victory.
It left Brighton to try and secure the first win for a Premier League club on the night, but they went down 5-4 on penalties to Barnet after the game ended in a 1-1 draw in north London.
Josh Robertson scored for the Seagulls in the 44th minute, with Callum Stead levelling the score from Ben Winterburn’s pass.
Harrogate Town beat Huddersfield Town 1-0 with Ellis Taylor rounding the keeper to seal their second win of the competition in a game which saw Bojan Radulović have an added-time leveller disdallowed after an interruption lasting several minutes because of a drone flying over the ground.