A sett on an unmade road in the West Wight was filled with concrete at some point on Friday, January 30.
The sett has collapsed, the Isle of Wight Badger Trust said, and the incident reported to police as a wildlife crime.
The local ecology officer has also been informed.
Graham Lee, from the IW Badger Trust, said: “This comes just five months after a police investigation at this same sett after two entrance holes were deliberately filled in.”
As previously reported by the County Press, police launched an investigation after active badger setts were ‘needlessly’ interfered with in August last year.
Badgers and their setts (tunnels and chambers where they live) are protected by law, under the Protection of Badgers Act 1992.
Filling in their setts carries a potential prison sentence and an unlimited fine.
Graham said there is a need for licensed work to put a large metal plate over the road, to safeguard the badger sett and to make sure the residents will still be able to drive their cars.
However, as it is breeding season, licensed work cannot be undertaken until after July 1, he said.
“The other problem – the unmade road has no registered owner with Land Registry, and residents don’t want to take responsibility for the costs of licensed work.
“Understandably it is a difficult situation for the residents, but there have been many years to get this sorted professionally, rather than keep on illegally interfering with the sett.”
A spokesperson for Hampshire and Isle of Wight Constabulary said: “We received reports around 9.13am yesterday (Saturday) of a badger sett being filled in with concrete in the Freshwater area.
“Enquiries are ongoing to establish the circumstances of the incident.”