The FBI said on Sunday that investigators recovered the glove from a field near the side of the road about two miles from Guthrie’s home in Tucson, Arizona.
The agency collected about 16 gloves from various locations near her home, though most turned out to have been discarded by people searching for her.
“The one with the DNA profile recovered is different and appears to match the gloves of the subject in the surveillance video,” the FBI said.
The agency said it has received preliminary DNA test results on the glove and is still waiting for “quality control and official confirmation” before entering an “unknown male profile” into its database.
Earlier this week, investigators released footage from a doorbell camera at Guthrie’s front door that shows a masked man wearing gloves and a backpack.
In the video, the suspect walks toward the camera and tries to cover it with his gloved hand before grabbing some brush from the front yard to block the camera’s view.
After conducting a forensic analysis of the video, the FBI announced earlier this week that it believed the man was between 5ft 9in and 5ft 10in (175-177cm) tall with an average build.
The FBI also highlighted the backpack the suspect was wearing in the video, identifying it as a 25-litre Ozark Trail Hiker Pack.