Octoputts at the Hardwick Industrial Estate in King’s Lynn had its final day of trading on Sunday.
The venue, opened by Natalie and Andy Atkins in 2023, boasted an 18-hole course with a range of nautical glow-in-the-dark obstacles.
The closure was announced earlier this month, with a spokesman citing difficult financial conditions as the reason the centre would be shutting its doors.
In a statement on Sunday, he added: “What started as a dream became something truly special because of all of you.
“To every family who visited, every laugh shared on the course and every person who supported us along the way, thank you.
“Many of you became more than customers. You became familiar faces we got to know and love.
“We will always be grateful for the memories we created together.”
The business stressed that Little Sharks soft play area would remain open, despite the closure of Octoputts.