The State Department said Rubio would work to “advance bilateral relations” with Italy and the Vatican and discuss “the situation in the Middle East and mutual interests in the Western Hemisphere”, including during meetings with other Italian counterparts.

On Tuesday ahead of his visit, Rubio denied that he was going to Italy to smooth over tensions between Trump and the Vatican.

“It’s a trip we have planned from before, and obviously we had some stuff that happened,” he said, adding there was “a lot to talk about with the Vatican”.

Earlier on Tuesday, Pope Leo said he would continue to call for peace despite criticisms.

“The mission of the Church is to preach the Gospel, to preach peace,” he said, according to Reuters. “If someone wants to criticise me for preaching the Gospel… I hope simply to be listened to because of the value of God’s words.”

Most recently, this week, Trump told conservative radio talkshow host Hugh Hewitt that the Pope was “endangering a lot of Catholics and a lot of people” because he “thinks it’s fine for Iran to have a nuclear weapon”. The Pope has not said he supports Iran having a nuclear weapon.

But Brian Burch, the US ambassador to the Holy See, told reporters this week that despite the recent tensions there was no “deep rift” between the US and the Vatican.

“Nations have disagreements, and I think one of the ways that you work through those is… through fraternity and authentic dialogue,” Burch told journalists.

“I don’t accept the idea that somehow there’s some deep rift,” he said, adding that Rubio’s meetings in Italy could allow the two sides to “talk through” any differences.

The disagreement also led to tensions between Trump and Italian Prime Minister Giorgia Meloni – a former Trump ally – as she also criticised the war in the Middle East and came to the defence of the Pope.

Asked by reporters whether she would meet Rubio this week, Meloni said: “I think so.”