The maned wolves have been living at the park for six months to acclimatise, and will now be living in areas open to visitors.
They have unusual proportions, with long legs that allow them to see over the long grasses of their natural habitat.
The species has been in decline in its native South America in recent decades.
“There are fewer than 17,000 maned wolves left in the wild and the species has seen a 20% reduction in just 15 years,” Kilbane said.
“This makes the three sisters incredibly important, even though we don’t plan to breed them currently.”