The herd has helped boost biodiversity by allowing more light into the forest through grazing, bark stripping and trampling.
Conservationists said they hope the herd can expand its range from 50 to 200 hectares, with the creation of the UK’s first bison bridge.
Selected bison will be moved to other rewilding sites to support genetic diversity and help establish further herds across the country, they added.
“The matriarch’s legacy goes far beyond this herd,” said Heidi Aguirregoicoa, Bison Ranger at Kent Wildlife Trust.
She said the Blean Bison project has created a blueprint for new approaches to conservation across the UK.
“Every change we’ve documented here – from woodland structure to species recovery to the regulatory changes we’re driving – now serves as a proven model for future rewilding efforts,” Ms Aguirregoicoa added.
The herd now consists of nine bison – five calves and four adults – with a new lead female emerging naturally.