Maya has nested at the Manton Bay site since 2009, and with male osprey 33 for the past 11 years.
Sexton, senior species and recording officer at Rutland Water, said the pair had raised 30 chicks during that time.
He said the recent egg, which he believed to be the last of the clutch of this season, was similar in colour to the first and third.
However, Sexton said the second was “completely different” in colour.
“Typically the eggs are reddy-brown speckled coloured, but the second was pale blue,” he said.
“We are not sure why that’s the case, but we are looking into it.”