The charity said donations made between 22 and 29 April would be doubled by the match-funding platform Big Give.
A charity spokesperson added: “At the turn of the 20th Century, 100,000 cheetahs roamed the wild; today, that number has plummeted to just 7,100.
“Now occupying only 9% of their historical range, cheetahs face an onslaught of threats, including escalating human-wildlife conflict, the illegal pet trade, and a dangerously shallow genetic pool.”
The cheetah is the smallest of the big cats, but is the world’s fastest land mammal.
The conservation facility would support the European Association of Zoos and Aquaria’s Southern Cheetah Ex-situ Programme to safeguard a healthy captive gene pool.