A spokesperson for developer Egdon Resources said a rig, about 30m high, would be used to decommission the bore.
The rig is expected to be on site for about a week and will not operate at night.
The spokesperson said the work would not cause any disruption to the community and the land would be returned to agricultural use.
The application for an oil well at Biscathorpe was initially refused by Lincolnshire County Council in 2021, but the developer successfully appealed against the decision at the High Court.
In 2024, an objection by SOS Biscathorpe led to a Supreme Court judgement which quashed the approval to drill and triggered a planning appeal hearing, which was due to be heard this month.
But the plans were abandoned in December, with the company behind the scheme, Union Jack Oil, saying it could “could no longer justify its continued investment”.