Untreated sewage poured into streets and gardens at East Cowes after a large pipe burst a week ago.
Following the burst Southern Water started discharging into the sea, for a total of more than eight hours, with the last release ending at 00.46 BST on Wednesday.
Repairs were completed to the burst sewage pipe in Millfield Avenue on Wednesday morning and the road reopened.
Signs warning swimmers of poor water quality were subsequently put up on the beaches by Isle of Wight Council.
The EA said in a previous statement: “As there is an ongoing impact on water quality, we have issued advice against bathing in the affected areas.
“We are continuing to investigate the incident and will not hesitate to take further action if required.”