The opening leg will start and finish in Lincoln, before heading to the coast for the second stage between Boston and Skegness.
The race then moves to Hull, before heading into the Wolds for the finish in Beverley, East Yorkshire.
North Yorkshire then hosts stage four, starting in Helmsley and finishing in Leyburn.
The final stage will see the peloton heading north over the border into Scotland, with a new start and finish location in the market town of Earlston for the first time in the event’s history.
Day added: “We are delighted to be bringing the Lloyds Tour of Britain Men to these fantastic locations.
“Taking the Tour to Lincoln for the first time, a city synonymous with its love for cycling, will be special for the opening stage of the men’s race.”
He added that Lincoln was known for hosting the annual Lincoln Grand Prix, which is the country’s longest‑running and biggest single-day road race and features a 656ft (200m) climb up the cobbles of Michaelgate towards the city’s cathedral.