Electric dipole moment drives the dynamics of the TNFR1 complex I signalosome
Micheau, O. & Tschopp, J. Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes. Cell 114,…
Structures of Marburgvirus glycoprotein and its complex with NPC1 receptor
Cell lines and plasmids HEK293T and Huh7 cells (American Type Culture Collection (ATCC)) were maintained in Dulbecco’s modified…
DICER cleavage fidelity is governed by 5′-end binding pockets
Plasmid construction The pXG-10×His-DICER plasmid was generated by inserting a DNA sequence encoding human DICER (amino acids 25–1922)…
Convergent MurJ flippase inhibition by phage lysis proteins
Murray, C. J. L. et al. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet…
Conformational diversity and fully opening mechanism of native NMDA receptor
Traynelis, S. F. et al. Glutamate receptor ion channels: structure, regulation, and function. Pharmacol. Rev. 62, 405–496 (2010).…
Targeting mGlyR with nanobodies for depression
Animals All animal experiments were approved by the Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology…
Synthetic α-synuclein fibrils replicate in mice causing MSA-like pathology
aSyn expression Escherichia coli strain BL21(DE3) was transformed with pT7-7 aSyn WT vector43 from Addgene, (plasmid 36046) and…
Structural snapshots capture nucleotide release at the μ-opioid receptor
Data reporting No statistical methods were used to predetermine sample size. The experiments were not randomized, and the…
Mechanism of conductance control and neurosteroid binding in NMDA receptors
Hille, B. Ion Channels of Excitable Membranes 3rd edn (Sinauer, 2001). Jahr, C. E. & Stevens, C. F.…
Helicase-mediated mechanism of SSU processome maturation and disassembly
Generation of endogenously tagged DHR1, KRE33, UTP7 and UTP14 strains Assembly intermediates of the S. cerevisiae SSU processomes…