Dissecting the impact of transcription factor dose on cell reprogramming heterogeneity using scTF-seq
Constructing the scTF-seq library and single-cell atlas To establish scTF-seq, we built a dox-inducible lentiviral open reading frame…
Large-scale visualization of α-synuclein oligomers in Parkinson’s disease brain tissue
Autofluorescence suppression and high-sensitivity microscopy reveals nanoscale assemblies in human brain tissue An overview of the ASA–PD pipeline…
CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes
Animals All mice were maintained according to the National Institutes of Health guidelines and all procedures used in…
Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states
Regev, A. et al. The human cell atlas. eLife 6, e27041 (2017). PubMed PubMed Central Google Scholar Karlsson,…
Methods and applications of in vivo CRISPR screening
Auton, A. et al. A global reference for human genetic variation. Nature 526, 68–74 (2015). Google Scholar GarcÃa-GarcÃa,…