RNA catalysis emerges from dynamic structural ensembles
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Mechanism of spliceosome termination | Nature
ILS disassembly assay in nematode extractSample preparation Total nematode extract was prepared from a C. elegans strain expressing…
Prion-like transmission of human tau strains in the mouse brain
Peng, C., Trojanowski, J. Q. & Lee, V. M.-Y. Protein transmission in neurodegenerative disease. Nat. Rev. Neurol. 16,…
Structural basis of transcription of the hachimoji eight-letter alphabet by E. coli RNA polymerase
Base pairing of 8-letter genetic alphabet in E. coli RNAP transcription The P:Z and B:S base pairs expand…
Imaging of nanoscale polar textures in quantum paraelectric SrTiO3
Cochran, W. Crystal stability and the theory of ferroelectricity. Adv. Phys. 9, 387–423 (1960). Article ADS CAS Google…
Structure of the pre-initiation complex explains CMGE biogenesis
Protein expression and purification HpaII methyltransferase (MH), ORC, Cdc6, Mcm2–7–Cdt1, DDK, CDK, (yeast-expressed) Sld3/7, Cdc45, GINS, (yeast-expressed) Pol ε,…
SNOR promotes translation restart after dormancy
Sample preparation for cryo-ET Cryo-ET samples were prepared using 200-mesh R2/2 copper grids (Quantifoil) plasma-cleaned for 30 s in…
Myosin forces remodel F-actin for mechanosensitive protein recognition
Unless otherwise noted, chemicals and buffer components were purchased from Sigma-Aldrich. Cell culture PtK2 cells (ATCC, CCL-56) were…
Electric dipole moment drives the dynamics of the TNFR1 complex I signalosome
Micheau, O. & Tschopp, J. Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes. Cell 114,…
Convergent MurJ flippase inhibition by phage lysis proteins
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Targeting mGlyR with nanobodies for depression
Animals All animal experiments were approved by the Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology…
Molecular mechanism of ultrafast transport by plasma membrane Ca2+-ATPases
Luan, S. & Wang, C. Calcium signaling mechanisms across kingdoms. Annu. Rev. Cell Dev. Biol. 37, 311–340 (2021).…